Anti-GENA, Rat-Mono(TRA98) (Cat. No. 73-003)
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Anti-GENA, Rat-Mono(TRA98) (Cat. No. 73-003)

Price: $300.00
  • Item #: 73-003
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Anti-GENA, Rat-Mono(TRA98)

Cat. No. 73-003

Store at -20°C

Size 50µg

Host Animal- Rat

Unlabeled 

Clone- TRA98

 

 

Description 

A monoclonal antibody TRA98 recognizes a mouse testicular germ cell-specific antigen (1). In adult tissues, bands of 60-100 kDa proteins are detected only in the testis by Western blotting analysis with TRA98 (Fig. 1). The signals are observed in male and female embryos after embryonic day 12.5. The signal in male is detected during development of germ cells and also after birth, but the signal in female disappears by 5 days after birth. The antigen is localized only in the nuclear fraction of testicular germ cells and this antibody can be used for immunohistochemical staining of testicular germ cells (1, 2). The hybridoma producing TRA98 was obtained by immunization of a rat with the cell lysate of adult mouse testis by the group of Prof. Yoshitake Nishimune of Osaka University (1). This product was purified from serum-free culture medium of the hybridoma clone TRA98 by proprietary chromatography processes under mild conditions.

 

References: This product has been used in the following publications.

1.Tanaka H et al ” A germ cell-specific nuclear antigen recognized by a monoclonal antibody raised against mouse testicular germ cells” Int J Androl 20: 361-366 (1997) PMID: 9568529

2. Ohta H et al “Regulation of proliferation and differentiation in spermatogonial stem cells: the role of c-kit and its ligand SCF” Development 127: 2125-2131 (2000) PMID: 10769236 

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