Anti-IgE Receptor, FcεR1α, CRA1, Human, Mouse-Mono(AER37),FITC
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Anti-IgE Receptor, FcεR1α, CRA1, Human, Mouse-Mono(AER37),FITC

Price: $345.00
  • Item #: 72-004
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Anti-IgE Receptor, FcεR1α, CRA1, Human, Mouse-Mono(AER37),FITC
Cat. No. 72-004
Store at -20°C
Size 50µg
Antigen- Human 
Host Animal- Mouse
Cross Reactivity- Human 
Application- FCM, IHC, Western Blot 
Label- FITC
Clone- AER37


 

 

Description 

FcεR1α is subunit of the high affinity receptor for IgE to which IgE directly binds. FcεR1 is a tetrameric complex consisting of one α, one β and two γ subunits. The latter two are required for signal transduction activity. The FcεR1 complex plays an important role in triggering allergic responses. The CRA1 (AER37) monoclonal antibody reacts with the FcεR1α subunit on a region that does not overlap the region of the IgE binding site, thus it does not compete with IgE for the receptor binding. Since the CRA2 (AER24) monoclonal antibody reacts with the IgE binding site on FcεR1α, it competes with IgE for the receptor binding. Combining the two antibodies, one can quantitatively measure the amounts of the IgE-bound FcεR1α. The IgG fraction was purified from serum free culture medium of mouse hybridoma (CRA1) by propriety chromatography under mild conditions. This product is an FITC-labeled IgG ([FITC]/[IgG] = 5.0) produced from the IgG fraction. 

 

References 

1. Ra C et al “A macrophage Fc gamma receptor and the mast cell receptor for IgE share an identical subunit”Nature 341:752-754 (1989) PMID: 2529442

2. Hakimi J et al “The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding” J Biol Chem 265:22079-22089 (1990) PMID: 2148316

3.Yamaguchi M et al ”IgE enhances Fc epsilon receptor I expression and IgE-dependent release of histamine and lipid mediators from human umbilical cord blood-derived mast cells: synergistic effect of IL-4 and IgE on human mast cell Fc epsilon receptor I expression and mediator release”J Immuno1 162:5455-5465 (1999) PMID: 10228025

4. Suzukawa M et al “IgE- and FcepsilonRI-mediated migration of human basophils” Int Immunol 17: 1249-1255 (2005) PMID: 16103029

5. Takai T et al “Epitope analysis and primary structures of variable regions of anti-human FcepsilonRI monoclonal antibodies, and expression of the chimeric antibodies fused with human constant regions” Biosci Biotechnol Biochem 64:1856-1867(2000) PMID: 11055388

6. Takai T et al “Direct expression of the extracellular portion of human FcepsilonRIalpha chain as inclusion bodies in Escherichia coli”Biosci Biotechnol Biochem 65:79-85 (2001) PMID: 11272849

7. Untersmayr E et al “The High Affinity IgE Receptor FceRI Is Expressed by Human Intestinal Epithelial Cells” PLoS ONE 5 (2):1-11 (2010) PLoS ONE: 0009023

* This product was used in references 3 -7.

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