Anti-IgE Receptor, FcεR1α, CRA2, Human, Mouse-Mono(AER24)
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Anti-IgE Receptor, FcεR1α, CRA2, Human, Mouse-Mono(AER24)

Price: $375.00
  • Item #: 72-005
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Anti-IgE Receptor, FcεR1α, CRA2, Human, Mouse-Mono(AER24)
Cat. No. 72-005
Store at -20°C
Size 100µg
Antigen- Human 
Host Animal- Mouse
Cross Reactivity- Human 
Application- FCM, IHC, Western Blot 
Unlabeled 
Purification- Pu
AER24
Class- IgG


 

 

Description 

FcεR1α is subunit of the high affinity receptor for IgE to which IgE directly binds. FcεR1α is a tetrameric complex consisting of one α, one β and two γ subunits. The latter two are required for signal transduction activity. The FcεR1 complex plays an important role in triggering allergic responses. The CRA2 (AER24) monoclonal antibody reacts with the FcεR1α subunit on a region that overlaps the region of the IgE binding site, thus it competes with IgE for the receptor binding. Since the CRA1 (AER37) monoclonal antibody reacts with the site different from the IgE binding site on FcεR1α, it does not compete with IgE for the receptor binding. Combining the two antibodies, one can quantitatively measure the amounts of the IgE-bound FcεR1α. This product is the IgG fraction purified from serum free culture medium of mouse hybridoma (CRA2) by propriety chromatography under mild conditions.

 

References 

1. Ra C et al “A macrophage Fc gamma receptor and the mast cell receptor for IgE share an identical subunit”Nature 341:752-754 (1989) PMID: 2529442

2. Hakimi J et al “The alpha subunit of the human IgE receptor (FcERI) is sufficient for high affinity IgE binding” J Biol Chem 265:22079-22089 (1990) PMID: 2148316

3. Takai T et al “Epitope analysis and primary structures of variable regions of anti-human FcepsilonRI monoclonal antibodies, and expression of the chimeric antibodies fused with human constant regions” Biosci Biotechnol Biochem 64:1856-1867(2000) PMID: 11055388

4. Takai T et al “Direct expression of the extracellular portion of human FcepsilonRIalpha chain as inclusion bodies in Escherichia coli ”Biosci Biotechnol Biochem 65:79-85 (2001) PMID: 11272849

5. Hasegawa S et al. “Functional Expression of the High Affinity Receptor for IgE (FceRI) in Human Platelets and Its’ Intracellular Expression in Human Megakaryocytes” Blood 93: 2543-2551 (1999) PMID: 10194433

6. Goto T et al. “ Enhanced expression of the high-affinity receptor for IgE (Fc(epsilon)RI) associated with decreased numbers of Langerhans cells in the lesional epidermis of atopic dermatitis” J Dermatol Sci. 27:156-61 (2001) PMID: 11641054

*This product was used in reference 3-6.

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