Anti-Rb(pSer795), Phosphorylated, Mouse-Mono(28B5)

Anti-Rb(pSer795), Phosphorylated, Mouse-Mono(28B5)

Cat. No. 71-171

Store at -20°C

Size 50µg

Antigen- Human

Host Animal- Mouse 

Cross Reactivity- Human

Application- ELISA, Western Blot

Unlabeled 

Purification- Pu

Clone- 28B5

Class IgG

Description 

Retinoblastoma protein (Rb), the tumor suppressor product of the retinoblastoma susceptibility gene, is a 110 kDa protein that functions as a negative regulator of the cell cycle by arresting cells in the G1 phase and halting inappropriate cell proliferation (1). At the transcriptional level, Rb protein exerts its growth suppressive function by binding to transcription factors including E2F1, PU1, ATF2, UBF, Elf-1 and c-Abl (2-4). Loss of Rb function leads to uncontrolled cell growth and tumor development and is found in all retinoblastomas and in a variety of other human malignancies. The ability of Rb protein to inhinbit transcription and cell cycle progression is inactivated by phosphorylation, which is catalyzed by the cyclin-dependent protein kinases. 

References

1. Sherr CJ “Cancer cell cycles”Science 274: 1672-1677 (1996) PMID:8939849

2. Nevins JR “E2F: a link between the Rb tumor suppressor protein and viral oncoproteins” Science 258: 424-429 (1992) PMID: 1411535

3. Welch PJ & Wang JY “A C-terminal protein-binding domain in the retinoblastoma protein regulates nuclear c-AbI tyrosine kinase in the cell cycle”Cell 75: 779-790 (1993) PMID: 8242749

4. Hu QJ et al ”The regions of the retinoblastoma protein needed for binding to adenovirus E1A or SV40 large T antigen are common sites for mutations”EMBO J 9:1147-1155 (1990) PMID: 2138977