Anti-SLA, Rat-Mono(TRA54) (Cat. No. 73-001)
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Anti-SLA, Rat-Mono(TRA54) (Cat. No. 73-001)

Price: $300.00
  • Item #: 73-001
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Anti-SLA, Rat-Mono(TRA54)

Cat. No. 73-001

Store at -20°C

Size 50µg

Host Animal- Rat

Unlabeled 

Clone- TRA54

 

 

Description 

Many transcripts for proteins specifically expressed in haploid germ cells are synthesized in the spermatogenesis. A monoclonal antibody TRA54 recognizes specific organelles in germ cell cytoplasm from spermatocytes to spermatids (1, 2). Western blots using TRA54 revealed broad bands with 85 kD, 190 kD and >200 kDa in testis probably due to alternative splicing and/or post-translational modification (Fig. 1). The molecular size diversity is due to the difference in glycosilation (Ref. 1). This antibody can be used for immunohistochemical staining of testicular tissues (1, 3). The hybridoma producing TRA54 was obtained from the immunaization of a rat with cell lysates of adult mouse testes by the group of Prof. Yoshitake Nishimune of Osaka University. 

 

References: Antibody TRA54 has been used in the following publications.

1. Pereira LA et al “Characterization and expression of a stage specific antigen by monoclonal antibody TRA 54 in testicular germ cell” Int J Androl 21: 34-40 (1998) PMID: 9639150 WB, IHC

2. Arrotéia KF et al “Identification and characterization of an antigen recognized by monoclonal antibody TRA 54 in mouse epididymis and vas deferens”J Androl 25: 914-921 (2004) PMID: 15477363 WB, IHC

3. Ventelä S et al ”Intercellular organelle traffic through cytoplasmic bridges in early spermatids of the rat: mechanisms of haploid gene product sharing” Mol Biol Cell 14: 2768-2780 (2003) PMID: 12857863 IHC, Immno-Electron Microscopy

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